• Human GPX1 (Glutathione peroxidase 1) Sandwich ELISA (STJE0003820)

Human GPX1 (Glutathione peroxidase 1) Sandwich ELISA (STJE0003820)

SKU:
STJE0003820

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This GPX1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GPX1
Gene ID: 2876
Uniprot ID: GPX1_HUMAN
Specificity: This kit recognizes Human GPX1 in samples. No significant cross-reactivity or interference between Human GPX1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.
Function Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism.
Protein Name Glutathione Peroxidase 1
Gpx-1
Gshpx-1
Cellular Glutathione Peroxidase
Database Links Reactome: R-HSA-2142688
Reactome: R-HSA-2142712
Reactome: R-HSA-2142770
Reactome: R-HSA-3299685
Cellular Localisation Cytoplasm
Alternative ELISA Names Glutathione Peroxidase 1 ELISA kit
Gpx-1 ELISA kit
Gshpx-1 ELISA kit
Cellular Glutathione Peroxidase ELISA kit
GPX1 ELISA kit
Specificity This kit recognizes Human GPX1 in samples. No significant cross-reactivity or interference between Human GPX1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 95
EDTA plasma (n=8) 86-98 91
Cell culture media (n=8) 86-100 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 110.99 204.30 946.17 118.12 214.76 1037.22
Standard deviation 5.67 8.52 51.85 6.08 9.96 44.29
CV (%) 5.11 4.17 5.48 5.15 4.64 4.27
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance