| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
2.35ng/mL |
| Detection Limit: |
3.91~250ng/mL |
| Short Description: |
This GC Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
GC |
| Gene ID: |
2638 |
| Uniprot ID: |
VTDB_HUMAN |
| Specificity: |
This kit recognizes Human DBP in samples. No significant cross-reactivity or interference between Human DBP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated. |
| Function | Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation. |
| Protein Name | Vitamin D-Binding ProteinDbpVdbGc Protein-Derived Macrophage Activating FactorGc-MafGcmafGc-GlobulinGroup-Specific ComponentGcVitamin D-Binding Protein-Macrophage Activating FactorDbp-Maf |
| Database Links | Reactome: R-HSA-196791 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Vitamin D-Binding Protein ELISA kitDbp ELISA kitVdb ELISA kitGc Protein-Derived Macrophage Activating Factor ELISA kitGc-Maf ELISA kitGcmaf ELISA kitGc-Globulin ELISA kitGroup-Specific Component ELISA kitGc ELISA kitVitamin D-Binding Protein-Macrophage Activating Factor ELISA kitDbp-Maf ELISA kitGC ELISA kit |
| Specificity | This kit recognizes Human DBP in samples. No significant cross-reactivity or interference between Human DBP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-98 | 92 |
| EDTA plasma (n=8) | 89-103 | 96 |
| Cell culture media (n=8) | 86-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 12.10 | 23.12 | 91.12 | 12.04 | 22.67 | 95.55 |
| Standard deviation | 0.70 | 1.17 | 3.78 | 0.69 | 1.09 | 5.17 |
| CV (%) | 5.79 | 5.06 | 4.15 | 5.73 | 4.81 | 5.41 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
