• Human GC (Vitamin D-binding protein) CLIA Kit (STJC0000233)

Human GC (Vitamin D-binding protein) CLIA Kit (STJC0000233)

SKU:
STJC0000233

£238.50 - £477.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This human GC kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: GC
Gene ID: 2638
Uniprot ID: VTDB_HUMAN
Specificity: This kit recognizes Human GC in samples. No significant cross-reactivity or interference between Human GC and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.
Post Translational Modifications Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated.
Function Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Protein Name Vitamin D-Binding Protein
Dbp
Vdb
Gc Protein-Derived Macrophage Activating Factor
Gc-Maf
Gcmaf
Gc-Globulin
Group-Specific Component
Gc
Vitamin D-Binding Protein-Macrophage Activating Factor
Dbp-Maf
Database Links Reactome: R-HSA-196791
Cellular Localisation Secreted
Alternative CLIA Names Vitamin D-Binding Protein CLIA kit
Dbp CLIA kit
Vdb CLIA kit
Gc Protein-Derived Macrophage Activating Factor CLIA kit
Gc-Maf CLIA kit
Gcmaf CLIA kit
Gc-Globulin CLIA kit
Group-Specific Component CLIA kit
Gc CLIA kit
Vitamin D-Binding Protein-Macrophage Activating Factor CLIA kit
Dbp-Maf CLIA kit
GC CLIA kit
Specificity This kit recognizes Human GC in samples. No significant cross-reactivity or interference between Human GC and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-106 97
EDTA plasma(n=8) 89-101 94
Cell culture media(n=8) 92-105 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 244.84 439.44 2344.03 246.94 396.12 2198.03
Standard deviation 30.43 46.98 184.24 20.32 36.56 137.60
CV (%) 12.43 10.69 7.86 8.23 9.23 6.26
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance