Human DBP (Vitamin D-binding protein) Sandwich ELISA (STJE0019757)

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STJE0019757

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This DBP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: An unopened kit can be stored at 2-8 °C for 1 week. If the kit is not supposed to be used within 1 week, store the items separately according to the following conditions once the kit is received.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GC
Gene ID: 2638
Uniprot ID: VTDB_HUMAN
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated.
Function Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Protein Name Vitamin D-Binding Protein
Dbp
Vdb
Gc Protein-Derived Macrophage Activating Factor
Gc-Maf
Gcmaf
Gc-Globulin
Group-Specific Component
Gc
Vitamin D-Binding Protein-Macrophage Activating Factor
Dbp-Maf
Database Links Reactome: R-HSA-196791
Cellular Localisation Secreted
Alternative ELISA Names Vitamin D-Binding Protein ELISA kit
Dbp ELISA kit
Vdb ELISA kit
Gc Protein-Derived Macrophage Activating Factor ELISA kit
Gc-Maf ELISA kit
Gcmaf ELISA kit
Gc-Globulin ELISA kit
Group-Specific Component ELISA kit
Gc ELISA kit
Vitamin D-Binding Protein-Macrophage Activating Factor ELISA kit
Dbp-Maf ELISA kit
GC ELISA kit
Specificity This kit recognizes Human DBP in samples. No significant cross-reactivity or interference between Human DBP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 91
EDTA plasma (n=8) 91-107 99
Cell culture media (n=8) 87-102 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.55 1.34 3.76 0.56 1.36 3.71
Standard deviation 0.04 0.08 0.15 0.03 0.07 0.16
CV (%) 6.84 5.84 4.09 5.36 5.32 4.39
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance