Applications: |
ELISA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
2.35ng/mL |
Detection Limit: |
3.91~250ng/mL |
Short Description: |
This GC Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Colormetric |
Gene Symbol: |
GC |
Gene ID: |
2638 |
Uniprot ID: |
VTDB_HUMAN |
Specificity: |
This kit recognizes Human DBP in samples. No significant cross-reactivity or interference between Human DBP and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Post Translational Modifications | Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated. |
Function | Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation. |
Protein Name | Vitamin D-Binding ProteinDbpVdbGc Protein-Derived Macrophage Activating FactorGc-MafGcmafGc-GlobulinGroup-Specific ComponentGcVitamin D-Binding Protein-Macrophage Activating FactorDbp-Maf |
Database Links | Reactome: R-HSA-196791 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Vitamin D-Binding Protein ELISA kitDbp ELISA kitVdb ELISA kitGc Protein-Derived Macrophage Activating Factor ELISA kitGc-Maf ELISA kitGcmaf ELISA kitGc-Globulin ELISA kitGroup-Specific Component ELISA kitGc ELISA kitVitamin D-Binding Protein-Macrophage Activating Factor ELISA kitDbp-Maf ELISA kitGC ELISA kit |
Specificity | This kit recognizes Human DBP in samples. No significant cross-reactivity or interference between Human DBP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 87-98 | 92 |
EDTA plasma (n=8) | 89-103 | 96 |
Cell culture media (n=8) | 86-99 | 92 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 12.10 | 23.12 | 91.12 | 12.04 | 22.67 | 95.55 |
Standard deviation | 0.70 | 1.17 | 3.78 | 0.69 | 1.09 | 5.17 |
CV (%) | 5.79 | 5.06 | 4.15 | 5.73 | 4.81 | 5.41 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance