Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
46.88pg/mL |
Detection Limit: |
78.13~5000pg/mL |
Short Description: |
This human GC kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
GC |
Gene ID: |
2638 |
Uniprot ID: |
VTDB_HUMAN |
Specificity: |
This kit recognizes Human GC in samples. No significant cross-reactivity or interference between Human GC and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Tissue Specificity | Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine. |
Post Translational Modifications | Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated. |
Function | Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation. |
Protein Name | Vitamin D-Binding ProteinDbpVdbGc Protein-Derived Macrophage Activating FactorGc-MafGcmafGc-GlobulinGroup-Specific ComponentGcVitamin D-Binding Protein-Macrophage Activating FactorDbp-Maf |
Database Links | Reactome: R-HSA-196791 |
Cellular Localisation | Secreted |
Alternative CLIA Names | Vitamin D-Binding Protein CLIA kitDbp CLIA kitVdb CLIA kitGc Protein-Derived Macrophage Activating Factor CLIA kitGc-Maf CLIA kitGcmaf CLIA kitGc-Globulin CLIA kitGroup-Specific Component CLIA kitGc CLIA kitVitamin D-Binding Protein-Macrophage Activating Factor CLIA kitDbp-Maf CLIA kitGC CLIA kit |
Specificity | This kit recognizes Human GC in samples. No significant cross-reactivity or interference between Human GC and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 91-106 | 97 |
EDTA plasma(n=8) | 89-101 | 94 |
Cell culture media(n=8) | 92-105 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 244.84 | 439.44 | 2344.03 | 246.94 | 396.12 | 2198.03 |
Standard deviation | 30.43 | 46.98 | 184.24 | 20.32 | 36.56 | 137.60 |
CV (%) | 12.43 | 10.69 | 7.86 | 8.23 | 9.23 | 6.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance