| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This FPN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SLC40A1 |
| Gene ID: |
30061 |
| Uniprot ID: |
S40A1_HUMAN |
| Specificity: |
This kit recognizes Human FPN in samples. No significant cross-reactivity or interference between Human FPN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Polyubiquitinated by RNF217.leading to proteasomal degradation. Ubiquitination is necessary for its internalization by hepcidin/HAMP. |
| Function | Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma. |
| Protein Name | Solute Carrier Family 40 Member 1Ferroportin-1Iron-Regulated Transporter 1 |
| Database Links | Reactome: R-HSA-425410Reactome: R-HSA-5619049Reactome: R-HSA-5619060Reactome: R-HSA-5655799Reactome: R-HSA-917937 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneLocalized To The Basolateral Membrane Of Hepatocytoma Wif-B Cells |
| Alternative ELISA Names | Solute Carrier Family 40 Member 1 ELISA kitFerroportin-1 ELISA kitIron-Regulated Transporter 1 ELISA kitSLC40A1 ELISA kitFPN ELISA kitFPN1 ELISA kitIREG1 ELISA kitSLC11A3 ELISA kitMSTP079 ELISA kit |
| Specificity | This kit recognizes Human FPN in samples. No significant cross-reactivity or interference between Human FPN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-96 | 90 |
| EDTA plasma (n=8) | 93-104 | 99 |
| Cell culture media (n=8) | 90-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.92 | 2.31 | 7.33 | 0.98 | 2.22 | 6.66 |
| Standard deviation | 0.05 | 0.12 | 0.23 | 0.06 | 0.10 | 0.29 |
| CV (%) | 5.43 | 5.19 | 3.14 | 6.12 | 4.50 | 4.35 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
