• Human GHRH (Somatoliberin) Sandwich ELISA (STJE0002750)
  • Human GHRH (Somatoliberin) Sandwich ELISA (STJE0002750)

Human GHRH (Somatoliberin) Sandwich ELISA (STJE0002750)

SKU:
STJE0002750

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This GHRH Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GHRH
Gene ID: 2691
Uniprot ID: SLIB_HUMAN
Specificity: This kit recognizes Human GHRH in samples. No significant cross-reactivity or interference between Human GHRH and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Somatoliberin
Growth Hormone-Releasing Factor
Grf
Growth Hormone-Releasing Hormone
Ghrh
Somatocrinin
Somatorelin
Sermorelin
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-420092
Cellular Localisation Secreted
Alternative ELISA Names Somatoliberin ELISA kit
Growth Hormone-Releasing Factor ELISA kit
Grf ELISA kit
Growth Hormone-Releasing Hormone ELISA kit
Ghrh ELISA kit
Somatocrinin ELISA kit
Somatorelin ELISA kit
Sermorelin ELISA kit
GHRH ELISA kit
GHRF ELISA kit
Specificity This kit recognizes Human GHRH in samples. No significant cross-reactivity or interference between Human GHRH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 92
EDTA plasma (n=8) 87-103 94
Cell culture media (n=8) 90-105 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.24 156.32 414.19 45.98 155.84 451.11
Standard deviation 2.71 9.29 22.24 2.64 8.06 14.57
CV (%) 5.74 5.94 5.37 5.74 5.17 3.23
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance