| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human FLNC kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FLNC |
| Gene ID: |
2318 |
| Uniprot ID: |
FLNC_HUMAN |
| Specificity: |
This kit recognizes Human FLNC in samples. No significant cross-reactivity or interference between Human FLNC and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highly expressed in striated muscles. Weakly expressed in thyroid, fetal brain, fetal lung, retina, spinal cord and bone marrow. Not expressed in testis, pancreas, adrenal gland, placenta, liver and kidney. |
| Post Translational Modifications | Ubiquitinated by FBXL22, leading to proteasomal degradation. |
| Function | Muscle-specific filamin, which plays a central role in sarcomere assembly and organization. Critical for normal myogenesis, it probably functions as a large actin-cross-linking protein with structural functions at the Z lines in muscle cells. May be involved in reorganizing the actin cytoskeleton in response to signaling events. |
| Protein Name | Filamin-CFln-CFlncAbp-280-Like ProteinAbp-LActin-Binding-Like ProteinFilamin-2Gamma-Filamin |
| Database Links | Reactome: R-HSA-446353 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinCytoskeletonMyofibrilSarcomereZ LineA Small Amount Localizes At MembranesIn Striated Muscle CellsIt Predominantly Localizes In Myofibrillar Z LinesWhile A Minor Fraction Localizes With SubsarcolemmeTargeting To Developing And Mature Z Lines Is Mediated By The Intradomain Insert |
| Alternative CLIA Names | Filamin-C CLIA kitFln-C CLIA kitFlnc CLIA kitAbp-280-Like Protein CLIA kitAbp-L CLIA kitActin-Binding-Like Protein CLIA kitFilamin-2 CLIA kitGamma-Filamin CLIA kitFLNC CLIA kitABPL CLIA kitFLN2 CLIA kit |
| Specificity | This kit recognizes Human FLNC in samples. No significant cross-reactivity or interference between Human FLNC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-97 | 91 |
| EDTA plasma(n=8) | 97-113 | 105 |
| Cell culture media(n=8) | 92-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 195.20 | 470.83 | 1377.23 | 204.46 | 485.28 | 1312.20 |
| Standard deviation | 20.57 | 42.09 | 92.55 | 17.81 | 46.59 | 138.17 |
| CV (%) | 10.54 | 8.94 | 6.72 | 8.71 | 9.60 | 10.53 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
