• Human FGF1 (Fibroblast growth factor 1) CLIA Kit (STJC0000066)

Human FGF1 (Fibroblast growth factor 1) CLIA Kit (STJC0000066)

SKU:
STJC0000066

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human FGF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: FGF1
Gene ID: 2246
Uniprot ID: FGF1_HUMAN
Specificity: This kit recognizes Human AFGF/FGF1 in samples. No significant cross-reactivity or interference between Human AFGF/FGF1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle.
Post Translational Modifications In the nucleus, phosphorylated by PKC/PRKCD.
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.
Protein Name Fibroblast Growth Factor 1
Fgf-1
Acidic Fibroblast Growth Factor
Afgf
Endothelial Cell Growth Factor
Ecgf
Heparin-Binding Growth Factor 1
Hbgf-1
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-1839122
Reactome: R-HSA-1839130
Reactome: R-HSA-190322
Reactome: R-HSA-190370
Reactome: R-HSA-190371
Reactome: R-HSA-190372
Reactome: R-HSA-190373
Reactome: R-HSA-190375
Reactome: R-HSA-190377
Reactome: R-HSA-2033519
Reactome: R-HSA-2219530
Reactome: R-HSA-5654219
Reactome: R-HSA-5654221
Reactome: R-HSA-5654227
Reactome: R-HSA-5654228
Reactome: R-HSA-5654687
Reactome: R-HSA-5654688
Reactome: R-HSA-5654689
Reactome: R-HSA-5654693
Reactome: R-HSA-5654695
Reactome: R-HSA-5654699
Reactome: R-HSA-5654700
Reactome: R-HSA-5654704
Reactome: R-HSA-5654706
Reactome: R-HSA-5654710
Reactome: R-HSA-5654712
Reactome: R-HSA-5654719
Reactome: R-HSA-5654720
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5655253
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-8851708
Cellular Localisation Secreted
Cytoplasm
Cell Cortex
Cytosol
Nucleus
Lacks A Cleavable Signal Sequence
Within The Cytoplasm
It Is Transported To The Cell Membrane And Then Secreted By A Non-Classical Pathway That Requires Cu(2+) Ions And S100a13
Secreted In A Complex With Syt1
Binding Of Exogenous Fgf1 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf1 Across Endosomal Membrane Into The Cytosol
Nuclear Import From The Cytosol Requires The Classical Nuclear Import Machinery
Involving Proteins Kpna1 And Kpnb1
As Well As Lrrc59
Alternative CLIA Names Fibroblast Growth Factor 1 CLIA kit
Fgf-1 CLIA kit
Acidic Fibroblast Growth Factor CLIA kit
Afgf CLIA kit
Endothelial Cell Growth Factor CLIA kit
Ecgf CLIA kit
Heparin-Binding Growth Factor 1 CLIA kit
Hbgf-1 CLIA kit
FGF1 CLIA kit
FGFA CLIA kit
Specificity This kit recognizes Human AFGF/FGF1 in samples. No significant cross-reactivity or interference between Human AFGF/FGF1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-111 103
EDTA plasma(n=8) 94-107 101
Cell culture media(n=8) 88-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 42.84 82.30 327.26 40.45 89.02 353.66
Standard deviation 4.36 6.32 28.01 4.91 8.46 37.84
CV (%) 10.18 7.68 8.56 12.14 9.50 10.70
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance