| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human FGFR1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FGFR1 |
| Gene ID: |
2260 |
| Uniprot ID: |
FGFR1_HUMAN |
| Specificity: |
This kit recognizes Human FGFR1 in samples. No significant cross-reactivity or interference between Human FGFR1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. |
| Post Translational Modifications | Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer and proceeds in a highly ordered manner. Initial autophosphorylation at Tyr-653 increases the kinase activity by a factor of 50 to 100. After this, Tyr-583 becomes phosphorylated, followed by phosphorylation of Tyr-463, Tyr-766, Tyr-583 and Tyr-585. In a third stage, Tyr-654 is autophosphorylated, resulting in a further tenfold increase of kinase activity. Phosphotyrosine residues provide docking sites for interacting proteins and so are crucial for FGFR1 function and its regulation. Ubiquitinated. FGFR1 is rapidly ubiquitinated by NEDD4 after autophosphorylation, leading to internalization and lysosomal degradation. CBL is recruited to activated FGFR1 via FRS2 and GRB2, and mediates ubiquitination and subsequent degradation of FGFR1. N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus. |
| Function | Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation. |
| Protein Name | Fibroblast Growth Factor Receptor 1Fgfr-1Basic Fibroblast Growth Factor Receptor 1BfgfrBfgf-R-1Fms-Like Tyrosine Kinase 2Flt-2N-SamProto-Oncogene C-FgrCd Antigen Cd331 |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-1839120Reactome: R-HSA-1839122Reactome: R-HSA-190370 P11362-19Reactome: R-HSA-190373 P11362-1Reactome: R-HSA-190374 P11362-1Reactome: R-HSA-2219530Reactome: R-HSA-375165 P11362-1Reactome: R-HSA-445144 P11362-1Reactome: R-HSA-5654219Reactome: R-HSA-5654687Reactome: R-HSA-5654688Reactome: R-HSA-5654689Reactome: R-HSA-5654693Reactome: R-HSA-5654726Reactome: R-HSA-5655302Reactome: R-HSA-5673001Reactome: R-HSA-6811558Reactome: R-HSA-8853336Reactome: R-HSA-9758919Reactome: R-HSA-9793380 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinNucleusCytoplasmCytosolCytoplasmic VesicleAfter Ligand BindingBoth Receptor And Ligand Are Rapidly InternalizedCan Translocate To The Nucleus After InternalizationOr By Translocation From The Endoplasmic Reticulum Or Golgi Apparatus To The CytosolAnd From There To The Nucleus |
| Alternative CLIA Names | Fibroblast Growth Factor Receptor 1 CLIA kitFgfr-1 CLIA kitBasic Fibroblast Growth Factor Receptor 1 CLIA kitBfgfr CLIA kitBfgf-R-1 CLIA kitFms-Like Tyrosine Kinase 2 CLIA kitFlt-2 CLIA kitN-Sam CLIA kitProto-Oncogene C-Fgr CLIA kitCd Antigen Cd331 CLIA kitFGFR1 CLIA kitBFGFR CLIA kitCEK CLIA kitFGFBR CLIA kitFLG CLIA kitFLT2 CLIA kitHBGFR CLIA kit |
| Specificity | This kit recognizes Human FGFR1 in samples. No significant cross-reactivity or interference between Human FGFR1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-102 | 93 |
| EDTA plasma(n=8) | 89-103 | 95 |
| Cell culture media(n=8) | 89-101 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 219.08 | 626.91 | 1688.77 | 205.04 | 624.69 | 1754.11 |
| Standard deviation | 21.29 | 55.04 | 135.61 | 17.55 | 50.16 | 188.04 |
| CV (%) | 9.72 | 8.78 | 8.03 | 8.56 | 8.03 | 10.72 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
