| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human DSP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
DSP |
| Gene ID: |
1832 |
| Uniprot ID: |
DESP_HUMAN |
| Specificity: |
This kit recognizes Human DSP in samples. No significant cross-reactivity or interference between Human DSP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in oral mucosa (at protein level). Expressed in arrector pili muscle (at protein level). Isoform DPI: Apparently an obligate constituent of all desmosomes. Isoform DPII: Resides predominantly in tissues and cells of stratified origin. |
| Function | Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance. |
| Protein Name | DesmoplakinDp250/210 Kda Paraneoplastic Pemphigus Antigen |
| Database Links | Reactome: R-HSA-351906Reactome: R-HSA-6798695Reactome: R-HSA-6805567Reactome: R-HSA-6809371Reactome: R-HSA-9696264Reactome: R-HSA-9696273 |
| Cellular Localisation | Cell JunctionDesmosomeCell MembraneLocalizes At The Intercalated Disk In Cardiomyocytes |
| Alternative CLIA Names | Desmoplakin CLIA kitDp CLIA kit250/210 Kda Paraneoplastic Pemphigus Antigen CLIA kitDSP CLIA kit |
| Specificity | This kit recognizes Human DSP in samples. No significant cross-reactivity or interference between Human DSP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-104 | 98 |
| EDTA plasma(n=8) | 93-107 | 101 |
| Cell culture media(n=8) | 98-113 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 209.51 | 451.06 | 1463.48 | 203.95 | 469.60 | 1495.10 |
| Standard deviation | 25.14 | 40.78 | 140.35 | 19.80 | 42.50 | 98.68 |
| CV (%) | 12.00 | 9.04 | 9.59 | 9.71 | 9.05 | 6.60 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
