• Human DSP (Desmoplakin) CLIA Kit (STJC0000807)

Human DSP (Desmoplakin) CLIA Kit (STJC0000807)

SKU:
STJC0000807

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human DSP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: DSP
Gene ID: 1832
Uniprot ID: DESP_HUMAN
Specificity: This kit recognizes Human DSP in samples. No significant cross-reactivity or interference between Human DSP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in oral mucosa (at protein level). Expressed in arrector pili muscle (at protein level). Isoform DPI: Apparently an obligate constituent of all desmosomes. Isoform DPII: Resides predominantly in tissues and cells of stratified origin.
Function Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance.
Protein Name Desmoplakin
Dp
250/210 Kda Paraneoplastic Pemphigus Antigen
Database Links Reactome: R-HSA-351906
Reactome: R-HSA-6798695
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Reactome: R-HSA-9696264
Reactome: R-HSA-9696273
Cellular Localisation Cell Junction
Desmosome
Cell Membrane
Localizes At The Intercalated Disk In Cardiomyocytes
Alternative CLIA Names Desmoplakin CLIA kit
Dp CLIA kit
250/210 Kda Paraneoplastic Pemphigus Antigen CLIA kit
DSP CLIA kit
Specificity This kit recognizes Human DSP in samples. No significant cross-reactivity or interference between Human DSP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-104 98
EDTA plasma(n=8) 93-107 101
Cell culture media(n=8) 98-113 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 209.51 451.06 1463.48 203.95 469.60 1495.10
Standard deviation 25.14 40.78 140.35 19.80 42.50 98.68
CV (%) 12.00 9.04 9.59 9.71 9.05 6.60
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance