| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human CXCL5 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CXCL5 |
| Gene ID: |
6374 |
| Uniprot ID: |
CXCL5_HUMAN |
| Specificity: |
This kit recognizes Human ENA-78/CXCL5 in samples. No significant cross-reactivity or interference between Human ENA-78/CXCL5 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. |
| Function | Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. |
| Protein Name | C-X-C Motif Chemokine 5Ena-78(1-78Epithelial-Derived Neutrophil-Activating Protein 78Neutrophil-Activating Peptide Ena-78Small-Inducible Cytokine B5 Cleaved Into - Ena-78(8-78 - Ena-78(9-78 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-X-C Motif Chemokine 5 CLIA kitEna-78(1-78 CLIA kitEpithelial-Derived Neutrophil-Activating Protein 78 CLIA kitNeutrophil-Activating Peptide Ena-78 CLIA kitSmall-Inducible Cytokine B5 Cleaved Into - Ena-78(8-78 - Ena-78(9-78 CLIA kitCXCL5 CLIA kitENA78 CLIA kitSCYB5 CLIA kit |
| Specificity | This kit recognizes Human ENA-78/CXCL5 in samples. No significant cross-reactivity or interference between Human ENA-78/CXCL5 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 83-99 | 90 |
| EDTA plasma(n=8) | 86-98 | 91 |
| Cell culture media(n=8) | 90-103 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 46.94 | 109.53 | 390.46 | 46.09 | 110.80 | 424.03 |
| Standard deviation | 3.78 | 7.86 | 26.75 | 4.02 | 11.72 | 47.02 |
| CV (%) | 8.05 | 7.18 | 6.85 | 8.72 | 10.58 | 11.09 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
