Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
18.75pg/mL |
Detection Limit: |
31.25~2000pg/mL |
Short Description: |
This human CTSB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
CTSB |
Gene ID: |
1508 |
Uniprot ID: |
CATB_HUMAN |
Specificity: |
This kit recognizes Human CTSB in samples. No significant cross-reactivity or interference between Human CTSB and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Tissue Specificity | Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum. |
Function | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis. |
Protein Name | Cathepsin BApp SecretaseAppsCathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain |
Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1679131Reactome: R-HSA-2022090Reactome: R-HSA-2132295Reactome: R-HSA-6798695 |
Cellular Localisation | LysosomeMelanosomeSecretedExtracellular SpaceApical Cell MembranePeripheral Membrane ProteinExtracellular SideIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocalizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells |
Alternative CLIA Names | Cathepsin B CLIA kitApp Secretase CLIA kitApps CLIA kitCathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain CLIA kitCTSB CLIA kitCPSB CLIA kit |
Specificity | This kit recognizes Human CTSB in samples. No significant cross-reactivity or interference between Human CTSB and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 98-111 | 104 |
EDTA plasma(n=8) | 101-116 | 108 |
Cell culture media(n=8) | 100-114 | 106 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 100.33 | 277.70 | 727.18 | 92.77 | 304.35 | 793.10 |
Standard deviation | 8.14 | 28.52 | 62.46 | 11.96 | 22.92 | 89.54 |
CV (%) | 8.11 | 10.27 | 8.59 | 12.89 | 7.53 | 11.29 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance