• Human CSF3 (Granulocyte colony-stimulating factor) Sandwich ELISA (STJE0001972)

Human CSF3 (Granulocyte colony-stimulating factor) Sandwich ELISA (STJE0001972)

SKU:
STJE0001972

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 23.44pg/mL
Detection Limit: 39.06~2500pg/mL
Short Description: This CSF3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CSF3
Gene ID: 1440
Uniprot ID: CSF3_HUMAN
Specificity: This kit recognizes Human G-CSF in samples. No significant cross-reactivity or interference between Human G-CSF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Protein Name Granulocyte Colony-Stimulating Factor
G-Csf
Pluripoietin
Filgrastim
Lenograstim
Database Links Reactome: R-HSA-449836
Reactome: R-HSA-6783783
Reactome: R-HSA-9674555
Reactome: R-HSA-9705462
Cellular Localisation Secreted
Alternative ELISA Names Granulocyte Colony-Stimulating Factor ELISA kit
G-Csf ELISA kit
Pluripoietin ELISA kit
Filgrastim ELISA kit
Lenograstim ELISA kit
CSF3 ELISA kit
C17orf33 ELISA kit
GCSF ELISA kit
Specificity This kit recognizes Human G-CSF in samples. No significant cross-reactivity or interference between Human G-CSF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-98 93
EDTA plasma (n=8) 86-98 93
Cell culture media (n=8) 84-100 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 124.90 276.50 1226.60 114.70 303.50 1183.60
Standard deviation 7.20 11.90 66.20 6.20 16.40 60.40
CV (%) 5.76 4.30 5.40 5.41 5.40 5.10
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance