| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human CSF3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CSF3 |
| Gene ID: |
1440 |
| Uniprot ID: |
CSF3_HUMAN |
| Specificity: |
This kit recognizes Human G-CSF in samples. No significant cross-reactivity or interference between Human G-CSF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). |
| Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. |
| Protein Name | Granulocyte Colony-Stimulating FactorG-CsfPluripoietinFilgrastimLenograstim |
| Database Links | Reactome: R-HSA-449836Reactome: R-HSA-6783783Reactome: R-HSA-9674555Reactome: R-HSA-9705462 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Granulocyte Colony-Stimulating Factor CLIA kitG-Csf CLIA kitPluripoietin CLIA kitFilgrastim CLIA kitLenograstim CLIA kitCSF3 CLIA kitC17orf33 CLIA kitGCSF CLIA kit |
| Specificity | This kit recognizes Human G-CSF in samples. No significant cross-reactivity or interference between Human G-CSF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-109 | 101 |
| EDTA plasma(n=8) | 93-108 | 98 |
| Cell culture media(n=8) | 83-97 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 37.35 | 101.99 | 383.49 | 37.71 | 104.98 | 353.05 |
| Standard deviation | 4.32 | 10.75 | 33.98 | 3.16 | 10.60 | 30.61 |
| CV (%) | 11.57 | 10.54 | 8.86 | 8.38 | 10.10 | 8.67 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
