| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Cripto-1/TDGF1-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Cripto at 2 Mu g/mL (100 Mu L/well) can bind Recombinant Human ALK4 with a linear range of 0.15-151 ng/mL. |
| Gene Symbol: |
CRIPTO |
| Gene ID: |
6997 |
| Uniprot ID: |
TDGF1_HUMAN |
| Immunogen Region: |
Leu31-Thr172 |
| Immunogen: |
Recombinant Human Cripto-1/TDGF1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu31-Thr172) of human Cripto/TDGF1 (Accession #NP_003203) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (TMEM8A), removing an acyl-chain at the sn-2 position of GPI and releasing CRIPTO as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (GPLD1) into a soluble form. |
| Function | GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. |
| Protein Name | Protein CriptoCripto - Egf-Cfc Family MemberCripto-1 Growth FactorCrgfEpidermal Growth Factor-Like Cripto Protein Cr1Teratocarcinoma-Derived Growth Factor 1 |
| Database Links | Reactome: R-HSA-1181150Reactome: R-HSA-1433617Reactome: R-HSA-2892247 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedReleased From The Cell Membrane By Gpi Cleavage |
| Alternative Protein Names | Protein Cripto proteinCripto - Egf-Cfc Family Member proteinCripto-1 Growth Factor proteinCrgf proteinEpidermal Growth Factor-Like Cripto Protein Cr1 proteinTeratocarcinoma-Derived Growth Factor 1 proteinCRIPTO proteinCRIPTO-1 proteinTDGF1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
