| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This CLU Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CLU |
| Gene ID: |
1191 |
| Uniprot ID: |
CLUS_HUMAN |
| Specificity: |
This kit recognizes Human CLU in samples. No significant cross-reactivity or interference between Human CLU and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Proteolytic cleavage is not necessary for its chaperone activity. All non-secreted forms are not proteolytically cleaved. Chaperone activity of uncleaved forms is dependent on a non-reducing environment. Polyubiquitinated, leading to proteasomal degradation. Under cellular stress, the intracellular level of cleaved form is reduced due to proteasomal degradation. Extensively glycosylated with sulfated N-linked carbohydrates. About 30% of the protein mass is comprised of complex N-linked carbohydrate. Endoplasmic reticulum (ER) stress induces changes in glycosylation status and increases level of hypoglycosylated forms. Core carbohydrates are essential for chaperone activity. Non-secreted forms are hypoglycosylated or unglycosylated. |
| Function | Isoform 1: Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. A mitochondrial form suppresses BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3. Plays a role in the clearance of immune complexes that arise during cell injury. Isoform 6: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Isoform 4: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Promotes cell death through interaction with BCL2L1 that releases and activates BAX. |
| Protein Name | ClusterinAging-Associated Gene 4 ProteinApolipoprotein JApo-JComplement Cytolysis InhibitorCliComplement-Associated Protein Sp-40 -40Ku70-Binding Protein 1Na1/Na2Sulfated Glycoprotein 2Sgp-2Testosterone-Repressed Prostate Message 2Trpm-2 Cleaved Into - Clusterin Beta ChainApojalphaComplement Cytolysis Inhibitor A ChainSp-40 -40 Beta-Chain - Clusterin Alpha ChainApojbetaComplement Cytolysis Inhibitor B ChainSp-40 -40 Alpha-Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-166665Reactome: R-HSA-6803157Reactome: R-HSA-977606 |
| Cellular Localisation | Isoform 1: SecretedCan Retrotranslocate From The Secretory Compartments To The Cytosol Upon Cellular StressIsoform 4: CytoplasmKeeps Cytoplasmic Localization In Stressed And Unstressed CellIsoform 6: CytoplasmNucleusCytoplasmMitochondrion MembranePeripheral Membrane ProteinCytoplasmic SideCytosolMicrosomeEndoplasmic ReticulumMitochondrionPerinuclear RegionCytoplasmic VesicleSecretory VesicleChromaffin GranuleSecreted Isoforms Can Retrotranslocate From The Secretory Compartments To The Cytosol Upon Cellular StressDetected In Perinuclear Foci That May Be Aggresomes Containing MisfoldedUbiquitinated ProteinsDetected At The Mitochondrion Membrane Upon Induction Of ApoptosisUnder Er StressA Immaturely Glycosylated Pre-Secreted Form Retrotranslocates From The Endoplasmic Reticulum (Er)-Golgi Network To The Cytoplasm To Localize In The Mitochondria Through Hspa5 InteractionEr Stress Reduces SecretionUnder The StressMinor Amounts Of Non-Secreted Forms Accumulate In CytoplasmNon-Secreted Forms Emerge Mainly From Failed TranslocationAlternative Splicing Or Non-Canonical Initiation Start Codon |
| Alternative ELISA Names | Clusterin ELISA kitAging-Associated Gene 4 Protein ELISA kitApolipoprotein J ELISA kitApo-J ELISA kitComplement Cytolysis Inhibitor ELISA kitCli ELISA kitComplement-Associated Protein Sp-40 -40 ELISA kitKu70-Binding Protein 1 ELISA kitNa1/Na2 ELISA kitSulfated Glycoprotein 2 ELISA kitSgp-2 ELISA kitTestosterone-Repressed Prostate Message 2 ELISA kitTrpm-2 Cleaved Into - Clusterin Beta Chain ELISA kitApojalpha ELISA kitComplement Cytolysis Inhibitor A Chain ELISA kitSp-40 -40 Beta-Chain - Clusterin Alpha Chain ELISA kitApojbeta ELISA kitComplement Cytolysis Inhibitor B Chain ELISA kitSp-40 -40 Alpha-Chain ELISA kitCLU ELISA kitAPOJ ELISA kitCLI ELISA kitKUB1 ELISA kitAAG4 ELISA kit |
| Specificity | This kit recognizes Human CLU in samples. No significant cross-reactivity or interference between Human CLU and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-109 | 101 |
| EDTA plasma (n=8) | 85-98 | 91 |
| Cell culture media (n=8) | 91-104 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 10.73 | 19.44 | 92.85 | 11.07 | 19.22 | 88.80 |
| Standard deviation | 0.62 | 0.83 | 3.00 | 0.58 | 0.96 | 4.37 |
| CV (%) | 5.78 | 4.27 | 3.23 | 5.24 | 4.99 | 4.92 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
