• Human CLU (Clusterin) Sandwich ELISA Kit (STJE0007256)

Human CLU (Clusterin) Sandwich ELISA Kit (STJE0007256)

SKU:
STJE0007256

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.37ng/mL
Detection Limit: 0.781-50ng/mL
Short Description: This CLU Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: CLU
Gene ID: 1191
Uniprot ID: CLUS_HUMAN
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Tissue Specificity Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
Post Translational Modifications Proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Proteolytic cleavage is not necessary for its chaperone activity. All non-secreted forms are not proteolytically cleaved. Chaperone activity of uncleaved forms is dependent on a non-reducing environment. Polyubiquitinated, leading to proteasomal degradation. Under cellular stress, the intracellular level of cleaved form is reduced due to proteasomal degradation. Extensively glycosylated with sulfated N-linked carbohydrates. About 30% of the protein mass is comprised of complex N-linked carbohydrate. Endoplasmic reticulum (ER) stress induces changes in glycosylation status and increases level of hypoglycosylated forms. Core carbohydrates are essential for chaperone activity. Non-secreted forms are hypoglycosylated or unglycosylated.
Function Isoform 1: Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. A mitochondrial form suppresses BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3. Plays a role in the clearance of immune complexes that arise during cell injury. Isoform 6: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Isoform 4: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Promotes cell death through interaction with BCL2L1 that releases and activates BAX.
Protein Name Clusterin
Aging-Associated Gene 4 Protein
Apolipoprotein J
Apo-J
Complement Cytolysis Inhibitor
Cli
Complement-Associated Protein Sp-40 -40
Ku70-Binding Protein 1
Na1/Na2
Sulfated Glycoprotein 2
Sgp-2
Testosterone-Repressed Prostate Message 2
Trpm-2 Cleaved Into - Clusterin Beta Chain
Apojalpha
Complement Cytolysis Inhibitor A Chain
Sp-40 -40 Beta-Chain - Clusterin Alpha Chain
Apojbeta
Complement Cytolysis Inhibitor B Chain
Sp-40 -40 Alpha-Chain
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-166665
Reactome: R-HSA-6803157
Reactome: R-HSA-977606
Cellular Localisation Isoform 1: Secreted
Can Retrotranslocate From The Secretory Compartments To The Cytosol Upon Cellular Stress
Isoform 4: Cytoplasm
Keeps Cytoplasmic Localization In Stressed And Unstressed Cell
Isoform 6: Cytoplasm
Nucleus
Cytoplasm
Mitochondrion Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytosol
Microsome
Endoplasmic Reticulum
Mitochondrion
Perinuclear Region
Cytoplasmic Vesicle
Secretory Vesicle
Chromaffin Granule
Secreted Isoforms Can Retrotranslocate From The Secretory Compartments To The Cytosol Upon Cellular Stress
Detected In Perinuclear Foci That May Be Aggresomes Containing Misfolded
Ubiquitinated Proteins
Detected At The Mitochondrion Membrane Upon Induction Of Apoptosis
Under Er Stress
A Immaturely Glycosylated Pre-Secreted Form Retrotranslocates From The Endoplasmic Reticulum (Er)-Golgi Network To The Cytoplasm To Localize In The Mitochondria Through Hspa5 Interaction
Er Stress Reduces Secretion
Under The Stress
Minor Amounts Of Non-Secreted Forms Accumulate In Cytoplasm
Non-Secreted Forms Emerge Mainly From Failed Translocation
Alternative Splicing Or Non-Canonical Initiation Start Codon
Alternative ELISA Names Clusterin ELISA kit
Aging-Associated Gene 4 Protein ELISA kit
Apolipoprotein J ELISA kit
Apo-J ELISA kit
Complement Cytolysis Inhibitor ELISA kit
Cli ELISA kit
Complement-Associated Protein Sp-40 -40 ELISA kit
Ku70-Binding Protein 1 ELISA kit
Na1/Na2 ELISA kit
Sulfated Glycoprotein 2 ELISA kit
Sgp-2 ELISA kit
Testosterone-Repressed Prostate Message 2 ELISA kit
Trpm-2 Cleaved Into - Clusterin Beta Chain ELISA kit
Apojalpha ELISA kit
Complement Cytolysis Inhibitor A Chain ELISA kit
Sp-40 -40 Beta-Chain - Clusterin Alpha Chain ELISA kit
Apojbeta ELISA kit
Complement Cytolysis Inhibitor B Chain ELISA kit
Sp-40 -40 Alpha-Chain ELISA kit
CLU ELISA kit
APOJ ELISA kit
CLI ELISA kit
KUB1 ELISA kit
AAG4 ELISA kit
output

Information sourced from Uniprot.org

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