| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CLC kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CLC |
| Gene ID: |
1178 |
| Uniprot ID: |
LEG10_HUMAN |
| Specificity: |
This kit recognizes Human CLC in samples. No significant cross-reactivity or interference between Human CLC and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed abundantly in the bone marrow. Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils. Expressed in CD25-positive regulatory T-cells (Treg) (at protein level). Found in intestinal tissue from patients with Celiac disease, expression is directly related to the histological grade of mucosal damage and to the number of eosinophils found in the duodenal lesion (at protein level). Found in sputum of patients with eosinophilic inflammatory diseases such as asthma (at protein level). |
| Function | Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg). |
| Protein Name | Galectin-10Gal-10Charcot-Leyden Crystal ProteinClcEosinophil LysophospholipaseLysolecithin Acylhydrolase |
| Cellular Localisation | CytoplasmCytosolCytoplasmic GranuleLocalized In Granules From Where It May Be Secreted Or Transported To Other Locations In The Cell |
| Alternative CLIA Names | Galectin-10 CLIA kitGal-10 CLIA kitCharcot-Leyden Crystal Protein CLIA kitClc CLIA kitEosinophil Lysophospholipase CLIA kitLysolecithin Acylhydrolase CLIA kitCLC CLIA kitLGALS10 CLIA kitLGALS10A CLIA kit |
| Specificity | This kit recognizes Human CLC in samples. No significant cross-reactivity or interference between Human CLC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-104 | 95 |
| EDTA plasma(n=8) | 89-103 | 96 |
| Cell culture media(n=8) | 94-106 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 102.59 | 225.87 | 935.82 | 112.07 | 246.17 | 927.38 |
| Standard deviation | 13.28 | 24.73 | 102.10 | 9.84 | 22.25 | 81.24 |
| CV (%) | 12.94 | 10.95 | 10.91 | 8.78 | 9.04 | 8.76 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
