| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human CGA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CGA |
| Gene ID: |
1081 |
| Uniprot ID: |
GLHA_HUMAN |
| Specificity: |
This kit recognizes Human CG Alpha in samples. No significant cross-reactivity or interference between Human CG Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways. |
| Protein Name | Glycoprotein Hormones Alpha ChainAnterior Pituitary Glycoprotein Hormones Common Subunit AlphaChoriogonadotropin Alpha ChainChorionic Gonadotrophin Subunit AlphaCg-AlphaFollicle-Stimulating Hormone Alpha ChainFsh-AlphaFollitropin Alpha ChainLuteinizing Hormone Alpha ChainLsh-AlphaLutropin Alpha ChainThyroid-Stimulating Hormone Alpha ChainTsh-AlphaThyrotropin Alpha Chain |
| Database Links | Reactome: R-HSA-193048Reactome: R-HSA-193993Reactome: R-HSA-209822Reactome: R-HSA-209968Reactome: R-HSA-375281Reactome: R-HSA-418555Reactome: R-HSA-8866910Reactome: R-HSA-975578 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Glycoprotein Hormones Alpha Chain CLIA kitAnterior Pituitary Glycoprotein Hormones Common Subunit Alpha CLIA kitChoriogonadotropin Alpha Chain CLIA kitChorionic Gonadotrophin Subunit Alpha CLIA kitCg-Alpha CLIA kitFollicle-Stimulating Hormone Alpha Chain CLIA kitFsh-Alpha CLIA kitFollitropin Alpha Chain CLIA kitLuteinizing Hormone Alpha Chain CLIA kitLsh-Alpha CLIA kitLutropin Alpha Chain CLIA kitThyroid-Stimulating Hormone Alpha Chain CLIA kitTsh-Alpha CLIA kitThyrotropin Alpha Chain CLIA kitCGA CLIA kit |
| Specificity | This kit recognizes Human CGα in samples. No significant cross-reactivity or interference between Human CGα and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 101 |
| EDTA plasma(n=8) | 83-95 | 90 |
| Cell culture media(n=8) | 92-105 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 48.83 | 157.13 | 366.66 | 52.43 | 158.27 | 339.99 |
| Standard deviation | 6.33 | 15.76 | 39.78 | 4.77 | 17.87 | 35.32 |
| CV (%) | 12.96 | 10.03 | 10.85 | 9.10 | 11.29 | 10.39 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
