Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
0.94ng/mL |
Detection Limit: |
1.56~100ng/mL |
Short Description: |
This human AHSG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
AHSG |
Gene ID: |
197 |
Uniprot ID: |
FETUA_HUMAN |
Specificity: |
This kit recognizes Human FETUA in samples. No significant cross-reactivity or interference between Human FETUA and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Tissue Specificity | Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. |
Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. O- and N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan at Asn-156: Hex5HexNAc4.N-glycan heterogeneity at Asn-176: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor). |
Function | Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. |
Protein Name | Alpha-2-Hs-GlycoproteinAlpha-2-Z-GlobulinBa-Alpha-2-GlycoproteinFetuin-A Cleaved Into - Alpha-2-Hs-Glycoprotein Chain A - Alpha-2-Hs-Glycoprotein Chain B |
Database Links | Reactome: R-HSA-114608Reactome: R-HSA-381426Reactome: R-HSA-6798695Reactome: R-HSA-8957275 |
Cellular Localisation | Secreted |
Alternative CLIA Names | Alpha-2-Hs-Glycoprotein CLIA kitAlpha-2-Z-Globulin CLIA kitBa-Alpha-2-Glycoprotein CLIA kitFetuin-A Cleaved Into - Alpha-2-Hs-Glycoprotein Chain A - Alpha-2-Hs-Glycoprotein Chain B CLIA kitAHSG CLIA kitFETUA CLIA kitPRO2743 CLIA kit |
Specificity | This kit recognizes Human FETUA in samples. No significant cross-reactivity or interference between Human FETUA and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 99-115 | 107 |
EDTA plasma(n=8) | 85-95 | 90 |
Cell culture media(n=8) | 94-107 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 5.37 | 16.07 | 42.83 | 5.13 | 17.63 | 41.57 |
Standard deviation | 0.51 | 1.17 | 3.95 | 0.48 | 2.00 | 3.69 |
CV (%) | 9.50 | 7.28 | 9.22 | 9.36 | 11.34 | 8.88 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance