| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This BMP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
BMP1 |
| Gene ID: |
649 |
| Uniprot ID: |
BMP1_HUMAN |
| Specificity: |
This kit recognizes Human BMP-1 in samples. No significant cross-reactivity or interference between Human BMP-1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Proteolytically activated in the trans-Golgi network by furin-like/paired basic proprotein convertases, cleavage is not required for secretion. |
| Function | Metalloprotease that plays key roles in regulating the formation of the extracellular matrix (ECM) via processing of various precursor proteins into mature functional enzymes or structural proteins. Thereby participates in several developmental and physiological processes such as cartilage and bone formation, muscle growth and homeostasis, wound healing and tissue repair. Roles in ECM formation include cleavage of the C-terminal propeptides from procollagens such as procollagen I, II and III or the proteolytic activation of the enzyme lysyl oxidase LOX, necessary to formation of covalent cross-links in collagen and elastic fibers. Additional substrates include matricellular thrombospondin-1/THBS1 whose cleavage leads to cell adhesion disruption and TGF-beta activation. Isoform BMP1-3: Plays an important role in bone repair by acting as a coactivator of BMP7. |
| Protein Name | Bone Morphogenetic Protein 1Bmp-1Mammalian Tolloid ProteinMtldProcollagen C-ProteinasePcp |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-1650814Reactome: R-HSA-2214320Reactome: R-HSA-2243919Reactome: R-HSA-8963896 P13497-3 |
| Cellular Localisation | Golgi ApparatusTrans-Golgi NetworkSecretedExtracellular SpaceExtracellular MatrixCo-Localizes With Postn In The GolgiIsoform Bmp1-3: Secreted |
| Alternative ELISA Names | Bone Morphogenetic Protein 1 ELISA kitBmp-1 ELISA kitMammalian Tolloid Protein ELISA kitMtld ELISA kitProcollagen C-Proteinase ELISA kitPcp ELISA kitBMP1 ELISA kitPCOLC ELISA kit |
| Specificity | This kit recognizes Human BMP-1 in samples. No significant cross-reactivity or interference between Human BMP-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-96 | 92 |
| EDTA plasma (n=8) | 96-106 | 102 |
| Cell culture media (n=8) | 83-95 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 41.34 | 106.75 | 420.88 | 47.90 | 102.62 | 402.75 |
| Standard deviation | 2.16 | 4.85 | 21.89 | 2.56 | 5.13 | 16.11 |
| CV (%) | 5.22 | 4.54 | 5.20 | 5.34 | 5.00 | 4.00 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
