| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This BECN1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
BECN1 |
| Gene ID: |
8678 |
| Uniprot ID: |
BECN1_HUMAN |
| Specificity: |
This kit recognizes Human BECN1 in samples. No significant cross-reactivity or interference between Human BECN1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy. In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy. Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages. 'Lys-11'-linked polyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. Polyubiquitinated at Lys-402 with 'Lys-48'-linkages. 'Lys-48'-linked polyubiquitination of Lys-402 leads to degradation. Deubiquitinated by ATXN3, leading to stabilization. Ubiquitinated at Lys-437 via 'Lys-63'-linkage by the DCX(AMBRA1) complex, thereby increasing the association between BECN1 and PIK3C3 to promote PIK3C3 activity. 'Lys-48'-linked ubiquitination by RNF216 leads to proteasomal degradation and autophagy inhibition. Proteolytically processed by caspases including CASP8 and CASP3.the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells. |
| Function | Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis.it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. |
| Protein Name | Beclin-1Coiled-Coil Myosin-Like Bcl2-Interacting ProteinProtein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5689880Reactome: R-HSA-9679504Reactome: R-HSA-9694676Reactome: R-HSA-9705671Reactome: R-HSA-983170 |
| Cellular Localisation | CytoplasmGolgi ApparatusTrans-Golgi Network MembranePeripheral Membrane ProteinEndosome MembraneEndoplasmic Reticulum MembraneMitochondrion MembraneEndosomeCytoplasmic VesicleAutophagosomeInteraction With Atg14 Promotes Translocation To AutophagosomesExpressed In Dendrites And Cell Bodies Of Cerebellar Purkinje CellsBeclin-1-C 35 Kda: MitochondrionNucleusBeclin-1-C 37 Kda: Mitochondrion |
| Alternative ELISA Names | Beclin-1 ELISA kitCoiled-Coil Myosin-Like Bcl2-Interacting Protein ELISA kitProtein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda ELISA kitBECN1 ELISA kitGT197 ELISA kit |
| Specificity | This kit recognizes Human BECN1 in samples. No significant cross-reactivity or interference between Human BECN1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-111 | 101 |
| EDTA plasma (n=8) | 93-106 | 101 |
| Cell culture media (n=8) | 93-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.49 | 1.15 | 4.39 | 0.53 | 1.05 | 4.42 |
| Standard deviation | 0.03 | 0.06 | 0.16 | 0.03 | 0.06 | 0.18 |
| CV (%) | 6.12 | 5.22 | 3.64 | 5.66 | 5.71 | 4.07 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
