• Human CHGB (Secretogranin-1) Sandwich ELISA (STJE0002512)
  • Human CHGB (Secretogranin-1) Sandwich ELISA (STJE0002512)

Human CHGB (Secretogranin-1) Sandwich ELISA (STJE0002512)

SKU:
STJE0002512

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This CHGB Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CHGB
Gene ID: 1114
Uniprot ID: SCG1_HUMAN
Specificity: This kit recognizes Human CHGB in samples. No significant cross-reactivity or interference between Human CHGB and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Extensively processed by limited proteolysis at conserved basic residues. Alternative processing are seen in different tissues. O-glycosylated.
Function Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Protein Name Secretogranin-1
Chromogranin-B
Cgb
Secretogranin I
Sgi Cleaved Into - Pe-11 - Gawk Peptide - Ccb Peptide
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Neuroendocrine And Endocrine Secretory Granules
Alternative ELISA Names Secretogranin-1 ELISA kit
Chromogranin-B ELISA kit
Cgb ELISA kit
Secretogranin I ELISA kit
Sgi Cleaved Into - Pe-11 - Gawk Peptide - Ccb Peptide ELISA kit
CHGB ELISA kit
SCG1 ELISA kit
Specificity This kit recognizes Human CHGB in samples. No significant cross-reactivity or interference between Human CHGB and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-101 96
EDTA plasma (n=8) 85-98 91
Cell culture media (n=8) 84-97 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 186.91 350.82 1795.25 180.09 319.24 1941.20
Standard deviation 12.60 17.12 80.07 11.38 15.96 89.30
CV (%) 6.74 4.88 4.46 6.32 5.00 4.60
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance