| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human BANP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
BANP |
| Gene ID: |
54971 |
| Uniprot ID: |
BANP_HUMAN |
| Specificity: |
This kit recognizes Human BANP in samples. No significant cross-reactivity or interference between Human BANP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Down-regulated in breast cancer cell lines. |
| Function | Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 activation, which causes cell cycle arrest. |
| Protein Name | Protein BanpBen Domain-Containing Protein 1Btg3-Associated Nuclear ProteinScaffold/Matrix-Associated Region-1-Binding Protein |
| Database Links | Reactome: R-HSA-6804759 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Protein Banp CLIA kitBen Domain-Containing Protein 1 CLIA kitBtg3-Associated Nuclear Protein CLIA kitScaffold/Matrix-Associated Region-1-Binding Protein CLIA kitBANP CLIA kitBEND1 CLIA kitSMAR1 CLIA kit |
| Specificity | This kit recognizes Human BANP in samples. No significant cross-reactivity or interference between Human BANP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-99 | 91 |
| EDTA plasma(n=8) | 99-114 | 107 |
| Cell culture media(n=8) | 95-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 98.20 | 194.12 | 696.97 | 105.50 | 200.85 | 691.98 |
| Standard deviation | 8.02 | 20.52 | 77.22 | 9.22 | 17.88 | 79.23 |
| CV (%) | 8.17 | 10.57 | 11.08 | 8.74 | 8.90 | 11.45 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
