| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
HIPK3 Positive Control for STJ501347 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:2, 500-1:5, 000 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
HIPK3 |
| Gene ID: |
10114 |
| Uniprot ID: |
HIPK3_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with HIPK3 antibody STJ501347. |
| Immunogen: |
Purified Protein |
| Tissue Specificity | Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung. |
| Post Translational Modifications | Autophosphorylated, but autophosphorylation is not required for catalytic activity. May be sumoylated. |
| Function | Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). |
| Peptide Name | Homeodomain-Interacting Protein Kinase 3Androgen Receptor-Interacting Nuclear Protein KinaseAnpkFas-Interacting Serine/Threonine-Protein KinaseFistHomolog Of Protein Kinase Yak1 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Peptide Names | Homeodomain-Interacting Protein Kinase 3 proteinAndrogen Receptor-Interacting Nuclear Protein Kinase proteinAnpk proteinFas-Interacting Serine/Threonine-Protein Kinase proteinFist proteinHomolog Of Protein Kinase Yak1 proteinHIPK3 proteinDYRK6 proteinFIST3 proteinPKY protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
