• Western blot analysis of extracts of SKOV3 cells, using ZP3 antibody (STJ110653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Anti-ZP3 antibody (170-390) (STJ110653)
  • Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody (STJ110653) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody (STJ110653) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody (STJ110653) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of SKOV3 cells, using ZP3 antibody (STJ110653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ZP3 antibody (170-390) (STJ110653)

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STJ110653

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZP3 (170-390) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZP3
Gene ID: 7784
Uniprot ID: ZP3_HUMAN
Immunogen Region: 170-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 170-390 of human ZP3 (NP_001103824.1).
Immunogen Sequence: LTFSLRLMEENWNAEKRSPT FHLGDAAHLQAEIHTGSHVP LRLFVDHCVATPTPDQNASP YHTIVDFHGCLVDGLTDASS AFKVPRPGPDTLQFTVDVFH FANDSRNMIYITCHLKVTLA EQDPDELNKACSFSKPSNSW FPVEGSADICQCCNKGDCGT PSHSRRQPHVMSQWSRSASR NRRHVTEEADVTVGPLIFLD RRGDHEVEQWALPSDTSVVL L
Tissue Specificity Expressed in oocytes (at protein level).
Post Translational Modifications Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. N-glycosylated. O-glycosylated.removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.
Function Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
Protein Name Zona Pellucida Sperm-Binding Protein 3
Sperm Receptor
Zp3a/Zp3b
Zona Pellucida Glycoprotein 3
Zp-3
Zona Pellucida Protein C Cleaved Into - Processed Zona Pellucida Sperm-Binding Protein 3
Database Links Reactome: R-HSA-2534343
Cellular Localisation Processed Zona Pellucida Sperm-Binding Protein 3: Zona Pellucida
Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Zona Pellucida Sperm-Binding Protein 3 antibody
Anti-Sperm Receptor antibody
Anti-Zp3a/Zp3b antibody
Anti-Zona Pellucida Glycoprotein 3 antibody
Anti-Zp-3 antibody
Anti-Zona Pellucida Protein C Cleaved Into - Processed Zona Pellucida Sperm-Binding Protein 3 antibody
Anti-ZP3 antibody
Anti-ZP3A antibody
Anti-ZP3B antibody
Anti-ZPC antibody

Information sourced from Uniprot.org

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