• Western blot analysis of extracts of various cell lines, using ZNF396 antibody (STJ115777) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 50s.

Anti-ZNF396 antibody (1-333) (STJ115777)

SKU:
STJ115777

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZNF396 (1-333) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZNF396
Gene ID: 252884
Uniprot ID: ZN396_HUMAN
Immunogen Region: 1-333
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ZNF396 (NP_665699.1).
Immunogen Sequence: MSAKLGKSSSLLTQTSEECN GILTEKMEEEEQTCDPDSSL HWSSSYSPETFRQQFRQFGY QDSPGPHEALSRLWELCHLW LRPEVHTKEQILELLVLEQF LAILPKELQAWVQKHHPENG EETVTMLEDVERELDGPKQI FFGRRKDMIAEKLAPSEITE ELPSSQLMPVKKQLQGASWE LQSLRPHDEDIKTTNVKSAS RQKTSLGIELHCNVSNILHM NGSQSSTYRGTYEQDGRFE
Tissue Specificity Expressed strongly in liver, moderately in skeletal muscle and weakly in kidney, pancreas, spleen and prostate.
Function Isoform 1 and isoform 2 act as DNA-dependent transcriptional repressors.
Protein Name Zinc Finger Protein 396
Zinc Finger And Scan Domain-Containing Protein 14
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Nucleus
Cytoplasm
Alternative Antibody Names Anti-Zinc Finger Protein 396 antibody
Anti-Zinc Finger And Scan Domain-Containing Protein 14 antibody
Anti-ZNF396 antibody
Anti-ZSCAN14 antibody

Information sourced from Uniprot.org

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