• Western blot analysis of extracts of various cell lines, using ZNF346 antibody (STJ115368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded rat brain using ZNF346 antibody (STJ115368) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human tonsil using ZNF346 antibody (STJ115368) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse brain using ZNF346 antibody (STJ115368) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of NIH/3T3 cells using ZNF346 antibody (STJ115368) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using ZNF346 antibody (STJ115368) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ZNF346 antibody (1-294) (STJ115368)

SKU:
STJ115368

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZNF346 (1-294) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZNF346
Gene ID: 23567
Uniprot ID: ZN346_HUMAN
Immunogen Region: 1-294
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-294 of human ZNF346 (NP_036411.1).
Immunogen Sequence: MEYPAPATVQAADGGAAGPY SSSELLEGQEPDGVRFDRER ARRLWEAVSGAQPVGREEVE HMIQKNQCLFTNTQCKVCCA LLISESQKLAHYQSKKHANK VKRYLAIHGMETLKGETKKL DSDQKSSRSKDKNQCCPICN MTFSSPVVAQSHYLGKTHAK NLKLKQQSTKVEALHQNREM IDPDKFCSLCHATFNDPVMA QQHYVGKKHRKQETKLKLMA RYGRLADPAVTDFPAGKGY
Function Binds with low affinity to dsDNA and ssRNA, and with high affinity to dsRNA, with no detectable sequence specificity. May bind to specific miRNA hairpins.
Protein Name Zinc Finger Protein 346
Just Another Zinc Finger Protein
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Nuclear At Steady State
Primarily In The Nucleolus
Shuttles Between The Nucleus And Cytoplasm When Associated With Xpo5
Alternative Antibody Names Anti-Zinc Finger Protein 346 antibody
Anti-Just Another Zinc Finger Protein antibody
Anti-ZNF346 antibody
Anti-JAZ antibody

Information sourced from Uniprot.org

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