• Western blot analysis of extracts of various cell lines, using GPR78 antibody (STJ114242) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPR78 antibody (294-363) (STJ114242)

SKU:
STJ114242

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPR78 (294-363) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPR78
Gene ID: 27201
Uniprot ID: GPR78_HUMAN
Immunogen Region: 294-363
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 294-363 of human GPR78 (NP_543009.2).
Immunogen Sequence: YSLLRRPFRQVLAGMVHRLL KRTPRPASTHDSSLDVAGMV HQLLKRTPRPASTHNGSVDT ENDSCLQQTH
Tissue Specificity High level of expression in placenta. Expressed throughout the brain at low level. No expression detected in skeletal muscle, lung, heart, liver, pancreas, or kidney.
Function Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP.
Protein Name G-Protein Coupled Receptor 78
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-G-Protein Coupled Receptor 78 antibody
Anti-GPR78 antibody
Anti-UNQ5925 antibody
Anti-PRO19818 antibody

Information sourced from Uniprot.org

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