• Western blot analysis of lysates from HeLa cells, using ZFYVE16 Rabbit polyclonal antibody (STJ115120) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ZFYVE16 antibody (1-300) (STJ115120)

SKU:
STJ115120

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZFYVE16 (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Zfyve16
Gene ID: 218441
Uniprot ID: ZFY16_MOUSE
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of mouse ZFYVE16 (NP_775568.1).
Immunogen Sequence: MDSYFKAAVSGLDKLLDDFE QNPDKQDYLPDAYAFNQCSV SSESASPQLALLSKDQRCIS TCASSEACCEDANETFLEGK IHEGLTSRPNEKNVAGLDLL SSVDASTSDEIQPSCMRRCS KPVCDLISDMGNLVHATNSE EDIKQLLPDDPKSSADTLIT LDSSSVSEALTVSSVDCGSN AVREEQNNINAGIKNRDISI KELGVKVDMALFDSCKYNRT ENLKDKIISNELETVDFDM
Function May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
Protein Name Zinc Finger Fyve Domain-Containing Protein 16
Endofin
Endosomal-Associated Fyve Domain Protein
Database Links Reactome: R-MMU-201451
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Localized To Early Endosomes
Membrane-Associated
Probably Via Its Association With Phosphatidylinositol 3-Phosphate (Pi3p)
Alternative Antibody Names Anti-Zinc Finger Fyve Domain-Containing Protein 16 antibody
Anti-Endofin antibody
Anti-Endosomal-Associated Fyve Domain Protein antibody
Anti-Zfyve16 antibody
Anti-Kiaa0305 antibody

Information sourced from Uniprot.org

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