• Western blot analysis of ULK3 Antibody. The lane on the right is blocked with the ULK3 peptide.
  • Immunohistochemistryt analysis of paraffin-embedded human breast carcinoma, using ULK3 Antibody. The lane on the right is blocked with the ULK3 peptide.
  • Western blot analysis of various cells using ULK3 Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Anti-ULK3 antibody (141-190 aa) (STJ96185)

SKU:
STJ96185

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Serine/threonine-protein kinase ULK3 (141-190 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ULK3
Gene ID: 25989
Uniprot ID: ULK3_HUMAN
Immunogen Region: 141-190 aa
Specificity: ULK3 Polyclonal Antibody detects endogenous levels of ULK3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ULK3 at the amino acid range 141-190
Post Translational Modifications Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU.
Function Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
Protein Name Serine/Threonine-Protein Kinase Ulk3
Unc-51-Like Kinase 3
Database Links Reactome: R-HSA-5632684
Cellular Localisation Cytoplasm
Localizes To Pre-Autophagosomal Structure During Cellular Senescence
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Ulk3 antibody
Anti-Unc-51-Like Kinase 3 antibody
Anti-ULK3 antibody

Information sourced from Uniprot.org

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