• Western blot analysis of extracts of various cell lines, using UGT1A4 antibody (STJ27495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-UGT1A4 antibody (29-150) (STJ27495)

SKU:
STJ27495

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UGT1A4 (29-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UGT1A4
Gene ID: 54657
Uniprot ID: UD14_HUMAN
Immunogen Region: 29-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-150 of human UGT1A4 (NP_009051.1).
Immunogen Sequence: GKVLVVPTDGSPWLSMREAL RELHARGHQAVVLTPEVNMH IKEEKFFTLTAYAVPWTQKE FDRVTLGYTQGFFETEHLLK RYSRSMAIMNNVSLALHRCC VELLHNEALIRHLNATSFDV VL
Tissue Specificity Isoform 1: Expressed in liver. Expressed in kidney, colon and small intestine. Not expressed in esophagus. Not expressed in skin. Isoform 2: Expressed in liver, kidney, colon, esophagus and small intestine.
Function Isoform 1: UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Involved in the glucuronidation of calcidiol, which is the major circulating form of vitamin D3 essential for the regulation of calcium and phosphate homeostasis. Also glucuronidates the biologically active form of vitamin D3, calcitriol, probably leading to its biliary transport and intestinal reabsorption. Isoform 2: Lacks UDP-glucuronosyltransferase (UGT) activity but acts as a negative regulator of isoform 1.
Protein Name Udp-Glucuronosyltransferase 1a4
Ugt1a4
Bilirubin-Specific Udpgt Isozyme 2
Hug-Br2
Udp-Glucuronosyltransferase 1-4
Udpgt 1-4
Ugt1*4
Ugt1-04
Ugt1.4
Udp-Glucuronosyltransferase 1-D
Ugt-1d
Ugt1d
Database Links Reactome: R-HSA-156588
Reactome: R-HSA-189483
Reactome: R-HSA-5579016
Reactome: R-HSA-9749641
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Udp-Glucuronosyltransferase 1a4 antibody
Anti-Ugt1a4 antibody
Anti-Bilirubin-Specific Udpgt Isozyme 2 antibody
Anti-Hug-Br2 antibody
Anti-Udp-Glucuronosyltransferase 1-4 antibody
Anti-Udpgt 1-4 antibody
Anti-Ugt1*4 antibody
Anti-Ugt1-04 antibody
Anti-Ugt1.4 antibody
Anti-Udp-Glucuronosyltransferase 1-D antibody
Anti-Ugt-1d antibody
Anti-Ugt1d antibody
Anti-UGT1A4 antibody
Anti-GNT1 antibody
Anti-UGT1 antibody

Information sourced from Uniprot.org

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