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Anti-TUBB3 antibody [5G3] (STJ96932)

IHC Staining of Human colon tissue, diluted at 1:200.
IF analysis of Hela with Beta-Tubulin monoclonal antibody (Left) and DAPI (Right) diluted at 1:100.
Immunofluorescence analysis of Mouse-lung tissue. 1, Beta-Tubulin monoclonal antibody (5G3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of A549 (1), Rat brain (2), Mouse brain (3), Chicken lung (4) and Rabbit testis (5), Sheep muscle (6), diluted at 1:5000.
Immunofluorescence analysis of Human-appendix tissue. 1, Beta-Tubulin monoclonal antibody (5G3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, Beta-Tubulin monoclonal antibody (5G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Beta-Tubulin monoclonal antibody (5G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, Beta-Tubulin monoclonal antibody (5G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1, CREB-1 (phospho Ser133) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-TUBB3 antibody [5G3] (STJ96932)

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STJ96932

€57.50 - €282.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Mouse
Applications:	WB/IF/IHC
Reactivity:	Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast/Fish/Cow/Rice
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Tubulin beta-3 chain is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.

Clonality:	Monoclonal
Clone ID:	5G3
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:5000 IHC 1:200 IF 1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	TUBB3
Gene ID:	10381
Uniprot ID:	TBB3_HUMAN
Specificity:	The antibody detects endogenous Beta-tubulin protein.
Immunogen:	Synthetic Peptide of Beta-Tubulin

Post Translational Modifications	Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Function	Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord.
Protein Name	Tubulin Beta-3 Chain Tubulin Beta-4 Chain Tubulin Beta-Iii
Database Links	Reactome: R-HSA-1445148 Reactome: R-HSA-190840 Reactome: R-HSA-190861 Reactome: R-HSA-2132295 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-3371497 Reactome: R-HSA-380320 Reactome: R-HSA-389957 Reactome: R-HSA-389960 Reactome: R-HSA-389977 Reactome: R-HSA-437239 Reactome: R-HSA-5610787 Reactome: R-HSA-5617833 Reactome: R-HSA-5620924 Reactome: R-HSA-5626467 Reactome: R-HSA-5663220 Reactome: R-HSA-6807878 Reactome: R-HSA-6811434 Reactome: R-HSA-6811436 Reactome: R-HSA-68877 Reactome: R-HSA-8852276 Reactome: R-HSA-8955332 Reactome: R-HSA-9609690 Reactome: R-HSA-9609736 Reactome: R-HSA-9619483 Reactome: R-HSA-9646399 Reactome: R-HSA-9648025 Reactome: R-HSA-9668328 Reactome: R-HSA-983189
Cellular Localisation	Cytoplasm Cytoskeleton Cell Projection Growth Cone Lamellipodium Filopodium
Alternative Antibody Names	Anti-Tubulin Beta-3 Chain antibody Anti-Tubulin Beta-4 Chain antibody Anti-Tubulin Beta-Iii antibody Anti-TUBB3 antibody Anti-TUBB4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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