| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TONSL (160-390) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TONSL |
| Gene ID: |
4796 |
| Uniprot ID: |
TONSL_HUMAN |
| Immunogen Region: |
160-390 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 160-390 of human TONSL (NP_038460.4). |
| Immunogen Sequence: |
MRTRLYLNLGLTFESLQQTA LCNDYFRKSIFLAEQNHLYE DLFRARYNLGTIHWRAGQHS QAMRCLEGARECAHTMRKRF MESECCVVIAQVLQDLGDFL AAKRALKKAYRLGSQKPVQR AAICQNLQHVLAVVRLQQQL EEAEGRDPQGAMVICEQLGD LFSKAGDFPRAAEAYQKQLR FAELLDRPGAERAIIHVSLA TTLGDMKDHHGAVRHYEEEL RLRSGNVLEEA |
| Tissue Specificity | Expressed in heart, skeletal muscle and tracheal epithelial cells. |
| Function | Component of the MMS22L-TONSL complex, a complex that promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication. It mediates the assembly of RAD51 filaments on single-stranded DNA (ssDNA): the MMS22L-TONSL complex is recruited to DSBs following histone replacement by histone chaperones and eviction of the replication protein A complex (RPA/RP-A) from DSBs. Following recruitment to DSBs, the TONSL-MMS22L complex promotes recruitment of RAD51 filaments and subsequent homologous recombination. Within the complex, TONSL acts as histone reader, which recognizes and binds newly synthesized histones following their replacement by histone chaperones. Specifically binds histone H4 lacking methylation at 'Lys-20' (H4K20me0) and histone H3.1. |
| Protein Name | Tonsoku-Like ProteinInhibitor Of Kappa B-Related ProteinI-Kappa-B-Related ProteinIkappabrNf-Kappa-B Inhibitor-Like Protein 2Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor-Like 2 |
| Cellular Localisation | NucleusChromosomeCytoplasmMainly NuclearLocalizes To Dna Damage SitesAccumulates At Stressed Replication ForksRecruited To Stalled Or Collapsed Replication Forks Following Histone Replacement By Histone Chaperones Asf1a And The Caf-1 Complex: Tonsl Acts As Histone Reader That Recognizes And Binds Newly Synthesized Histones |
| Alternative Antibody Names | Anti-Tonsoku-Like Protein antibodyAnti-Inhibitor Of Kappa B-Related Protein antibodyAnti-I-Kappa-B-Related Protein antibodyAnti-Ikappabr antibodyAnti-Nf-Kappa-B Inhibitor-Like Protein 2 antibodyAnti-Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor-Like 2 antibodyAnti-TONSL antibodyAnti-IKBR antibodyAnti-NFKBIL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
