| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TMEM59 (36-230) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TMEM59 |
| Gene ID: |
9528 |
| Uniprot ID: |
TMM59_HUMAN |
| Immunogen Region: |
36-230 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 36-230 of human TMEM59 (NP_004863.2). |
| Immunogen Sequence: |
EAFDSVLGDTASCHRACQLT YPLHTYPKEEELYACQRGCR LFSICQFVDDGIDLNRTKLE CESACTEAYSQSDEQYACHL GCQNQLPFAELRQEQLMSLM PKMHLLFPLTLVRSFWSDMM DSAQSFITSSWTFYLQADDG KIVIFQSKPEIQYAPHLEQE PTNLRESSLSKMSYLQMRNS QAHRNFLEDGESDGF |
| Post Translational Modifications | N-glycosylated. |
| Function | Acts as a regulator of autophagy in response to S.aureus infection by promoting activation of LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). Acts by interacting with ATG16L1, leading to promote a functional complex between LC3 and ATG16L1 and promoting LC3 lipidation and subsequent activation of autophagy. Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP. Inhibits APP transport to the cell surface and further shedding. |
| Protein Name | Transmembrane Protein 59Liver Membrane-Bound Protein |
| Database Links | Reactome: R-HSA-9013407Reactome: R-HSA-9696273 |
| Cellular Localisation | Late Endosome MembraneSingle-Pass Type I Membrane ProteinLysosome MembraneCell MembraneGolgi Apparatus MembraneMainly Localizes To Late Endosomes/LysosomesProbably First Exported To The Cell Surface And Then Actively Endocytosed To Transiently Localize In Early Endosomes On Its Way To The Late Endosomal/Lysosomal Compartment Where It Becomes Quickly Degraded |
| Alternative Antibody Names | Anti-Transmembrane Protein 59 antibodyAnti-Liver Membrane-Bound Protein antibodyAnti-TMEM59 antibodyAnti-C1orf8 antibodyAnti-HSPC001 antibodyAnti-UNQ169 antibodyAnti-PRO195 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89103/357560/STJ11100031_1__14868.1713121685.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
