• Immunofluorescence analysis of NIH-3T3 cells using TMED10 Rabbit polyclonal antibody (STJ11104294) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TMED10 in paraffin-embedded human liver damage using TMED10 Rabbit polyclonal antibody (STJ11104294) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TMED10 in paraffin-embedded mouse brain using TMED10 Rabbit polyclonal antibody (STJ11104294) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TMED10 in paraffin-embedded rat kidney using TMED10 Rabbit polyclonal antibody (STJ11104294) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TMED10 Rabbit polyclonal antibody (STJ11104294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TMED10 antibody (35-190) (STJ11104294)

SKU:
STJ11104294

Current Stock:
Host: Rabbit
Applications: WB/IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transmembrane Emp24 Domain-Containing Protein 10 (35-190) is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TMED10
Gene ID: 10972
Uniprot ID: TMEDA_HUMAN
Immunogen Region: 35-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-190 of human TMED10 (NP_006818.3).
Immunogen Sequence: HLPINSRKCLREEIHKDLLV TGAYEISDQSGGAGGLRSHL KITDSAGHILYSKEDATKGK FAFTTEDYDMFEVCFESKGT GRIPDQLVILDMKHGVEAKN YEEIAKVEKLKPLEVELRRL EDLSESIVNDFAYMKKREEE MRDTNESTNTRVLYFS
Function Cargo receptor involved in protein vesicular trafficking and quality control in the endoplasmic reticulum (ER) and Golgi. The p24 protein family is a group of transmembrane proteins that bind coat protein complex I/COPI and coat protein complex II/COPII involved in vesicular trafficking between the membranes. Acts at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and involved in vesicle coat formation at the cytoplasmic side. Mainly functions in the early secretory pathway and cycles between the ER, ER-Golgi intermediate compartment (ERGIC) and Golgi, mediating cargo transport through COPI and COPII-coated vesicles. In COPII vesicle-mediated anterograde transport, involved in the transport of GPI-anchored proteins by acting together with TMED2 as their cargo receptor.the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by the GPI inositol-deacylase/PGAP1 and the metallophosphoesterase MPPE1/PGAP5. In COPI vesicle-mediated retrograde transport, involved in the biogenesis of COPI vesicles and vesicle coat recruitment. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent from the modulation of gamma-secretase activity). Involved in the KDELR2-mediated retrograde transport of the toxin A subunit (CTX-A-K63)together with COPI and the COOH terminus of KDELR2. On Golgi membranes, acts as primary receptor for ARF1-GDP, a GTP-binding protein involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2 which mediates the hydrolysis of ARF1-bound GTP and therefore modulates protein trafficking from the Golgi apparatus. Involved in the exocytic trafficking of G protein-coupled receptors F2LR1/PAR2 (trypsin and tryspin-like enzyme receptor), OPRM1 (opioid receptor) and P2RY4 (UTD and UDP receptor) from the Golgi to the plasma membrane, thus contributing to receptor resensitization. In addition to its cargo receptor activity, may also act as a protein channel after oligomerization, facilitating the post-translational entry of leaderless cytoplasmic cargo into the ERGIC. Involved in the translocation into ERGIC, the vesicle entry and the secretion of leaderless cargos (lacking the secretion signal sequence), including the mature form of interleukin 1/IL-1 family members, the alpha-crystallin B chain HSPB5, the carbohydrate-binding proteins galectin-1/LGALS1 and galectin-3/LGALS3, the microtubule-associated protein Tau/MAPT, and the annexin A1/ANXA1.the translocation process is dependent on cargo protein unfolding and enhanced by chaperones HSP90AB1 and HSP90B1/GRP9. Could also associates with the presenilin-dependent gamma-secretase complex in order to regulate gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40/Abeta40.
Protein Name Transmembrane Emp24 Domain-Containing Protein 10
Protein Tmed10
21 Kda Transmembrane-Trafficking Protein
S31i125
S31iii125
Tmp-21-I
Transmembrane Protein Tmp21
P23
P24 Family Protein Delta-1
P24delta1
P24delta
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-5694530
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Golgi Apparatus Membrane
Golgi Apparatus
Cis-Golgi Network Membrane
Trans-Golgi Network Membrane
Cytoplasmic Vesicle
Secretory Vesicle Membrane
Cell Membrane
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Transmembrane Emp24 Domain-Containing Protein 10 antibody
Anti-Protein Tmed10 antibody
Anti-21 Kda Transmembrane-Trafficking Protein antibody
Anti-S31i125 antibody
Anti-S31iii125 antibody
Anti-Tmp-21-I antibody
Anti-Transmembrane Protein Tmp21 antibody
Anti-P23 antibody
Anti-P24 Family Protein Delta-1 antibody
Anti-P24delta1 antibody
Anti-P24delta antibody
Anti-TMED10 antibody
Anti-TMP21 antibody

Information sourced from Uniprot.org

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