• Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of MCF7 cells using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from 293F cells using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TJP1 antibody (1500-1650) (STJ11105590)

SKU:
STJ11105590

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tight Junction Protein Zo-1 (1500-1650) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TJP1
Gene ID: 7082
Uniprot ID: ZO1_HUMAN
Immunogen Region: 1500-1650
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1500-1650 of human ZO-1 (NP_003248.3).
Immunogen Sequence: PQKSFPDKAPVNGTEQTQKT VTPAYNRFTPKPYTSSARPF ERKFESPKFNHNLLPSETAH KPDLSSKTPTSPKTLVKSHS LAQPPEFDSGVETFSIHAEK PKYQINNISTVPKAIPVSPS AVEEDEDEDGHTVVATARGI FNSNGGVLSSI
Tissue Specificity The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
Post Translational Modifications Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ.
Function TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus.
Protein Name Tight Junction Protein Zo-1
Tight Junction Protein 1
Zona Occludens Protein 1
Zonula Occludens Protein 1
Database Links Reactome: R-HSA-191650
Reactome: R-HSA-2028269
Reactome: R-HSA-351906
Reactome: R-HSA-8935964
Reactome: R-HSA-9705677
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Junction
Tight Junction
Gap Junction
Cell Projection
Podosome
Moves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell Contact
At Podosomal Sites
Is Predominantly Localized In The Ring Structure Surrounding The Actin Core
Colocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells
Alternative Antibody Names Anti-Tight Junction Protein Zo-1 antibody
Anti-Tight Junction Protein 1 antibody
Anti-Zona Occludens Protein 1 antibody
Anti-Zonula Occludens Protein 1 antibody
Anti-TJP1 antibody
Anti-ZO1 antibody

Information sourced from Uniprot.org

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