| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-THOC5 (150-370) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
THOC5 |
| Gene ID: |
8563 |
| Uniprot ID: |
THOC5_HUMAN |
| Immunogen Region: |
150-370 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 150-370 of human THOC5 (NP_003669.4). |
| Immunogen Sequence: |
FKSKHEEIDLVSLEEFYKEA PPDISKAEVTMGDPHQQTLA RLDWELEQRKRLAEKYRECL SNKEKILKEIEVKKEYLSSL QPRLNSIMQASLPVQEYLFM PFDQAHKQYETARHLPPPLY VLFVQATAYGQACDKTLSVA IEGSVDEAKALFKPPEDSQD DESDSDAEEEQTTKRRRPTL GVQLDDKRKEMLKRHPLSVM LDLKCKDDSVLHLTFYYLMN L |
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Phosphorylated on tyrosine upon binding to activated CSF1R.which causes a dissociation of the two proteins. Phosphorylation on Ser-5 and/or Ser-6 is required for nuclear export. Phosphorylated on Thr-328 in insulin-stimulated adipocytes. Phosphorylation at Tyr-225 modulates mRNA binding. |
| Function | Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA.both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes.probably mediates association of the TREX and CFIm complexes. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development. |
| Protein Name | Tho Complex Subunit 5 HomologFunctional Spliceosome-Associated Protein 79Fsap79Nf2/Meningioma Region Protein Pk1.3Placental Protein 39.2Pp39.2Htrex90 |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72187Reactome: R-HSA-73856Reactome: R-HSA-9680350 |
| Cellular Localisation | NucleusCytoplasmShuttles Between Nucleus And Cytoplasm |
| Alternative Antibody Names | Anti-Tho Complex Subunit 5 Homolog antibodyAnti-Functional Spliceosome-Associated Protein 79 antibodyAnti-Fsap79 antibodyAnti-Nf2/Meningioma Region Protein Pk1.3 antibodyAnti-Placental Protein 39.2 antibodyAnti-Pp39.2 antibodyAnti-Htrex90 antibodyAnti-THOC5 antibodyAnti-C22orf19 antibodyAnti-KIAA0983 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
