• Western blot analysis of lysates from mESCs cells, using TET3 Rabbit polyclonal antibody (STJ119440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TET3 antibody (1551-1650) (STJ119440)

SKU:
STJ119440

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TET3 (1551-1650) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TET3
Gene ID: 200424
Uniprot ID: TET3_HUMAN
Immunogen Region: 1551-1650
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1551-1650 of human TET3 (NP_001274420.1).
Immunogen Sequence: GFQDKLWNPMKGEEGRIPAA GASQLDRAWQSFGLPLGSSE KLFGALKSEEKLWDPFSLEE GPAEEPPSKGAVKEEKGGGG AEEEEEELWSDSEHNFLDEN
Tissue Specificity Expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes.
Post Translational Modifications Monoubiquitinated at Lys-994 by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(VprBP) or CUL4A-RBX1-DDB1-DCAF1/VPRBP complex.this modification promotes binding to DNA.
Function Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG.
Protein Name Methylcytosine Dioxygenase Tet3
Database Links Reactome: R-HSA-5221030
Cellular Localisation Nucleus
Cytoplasm
Chromosome
At The Zygotic Stage
Localizes In The Male Pronucleus
While It Localizes To The Cytoplasm At Other Preimplantation Stages
Binds To The Promoter Of Target Genes
Close To The Transcription Start Site
Alternative Antibody Names Anti-Methylcytosine Dioxygenase Tet3 antibody
Anti-TET3 antibody
Anti-KIAA0401 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance