| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TCF25 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TCF25 |
| Gene ID: |
22980 |
| Uniprot ID: |
TCF25_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TCF25 (NP_055787.1). |
| Immunogen Sequence: |
MSRRALRRLRGEQRGQEPLG PGALHFDLRDDDDAEEEGPK RELGVRRPGGAGKEGVRVNN RFELINIDDLEDDPVVNGER SGCALTDAVAPGNKGRGQRG NTESKTDGDDTETVPSEQSH ASGKLRKKKKKQKNKKSSTG EASENGLEDIDRILERIEDS TGLNRPGPAPLSSRKHVLYV EHRHLNPDTELKRYFGARAI LGEQRPRQRQRVYPKCTWLT TPKSTWPRYSKPGLSMRLL |
| Tissue Specificity | In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart. |
| Function | Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. In the RQC complex, required to promote formation of 'Lys-48'-linked polyubiquitin chains during ubiquitination of incompletely synthesized proteins by LTN1. Also acts as a transcriptional repressor: represses transcription of SRF in vitro and so may play a role in heart development. May play a role in cell death control. |
| Protein Name | Ribosome Quality Control Complex Subunit Tcf25Nuclear Localized Protein 1Transcription Factor 25Tcf-25 |
| Cellular Localisation | NucleusCytoplasmCytosolMainly Nuclear |
| Alternative Antibody Names | Anti-Ribosome Quality Control Complex Subunit Tcf25 antibodyAnti-Nuclear Localized Protein 1 antibodyAnti-Transcription Factor 25 antibodyAnti-Tcf-25 antibodyAnti-TCF25 antibodyAnti-KIAA1049 antibodyAnti-NULP1 antibodyAnti-FKSG26 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
