Western blot analysis of lysates from U-87MG cells, using TCF25 Rabbit polyclonal antibody (STJ117334) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TCF25 antibody (1-300) (STJ117334)

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STJ117334
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TCF25
Gene ID: 22980
Uniprot ID: TCF25_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TCF25 (NP_055787.1).
Immunogen Sequence: MSRRALRRLRGEQRGQEPLG PGALHFDLRDDDDAEEEGPK RELGVRRPGGAGKEGVRVNN RFELINIDDLEDDPVVNGER SGCALTDAVAPGNKGRGQRG NTESKTDGDDTETVPSEQSH ASGKLRKKKKKQKNKKSSTG EASENGLEDIDRILERIEDS TGLNRPGPAPLSSRKHVLYV EHRHLNPDTELKRYFGARAI LGEQRPRQRQRVYPKCTWLT TPKSTWPRYSKPGLSMRLL
Tissue Specificity In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart.
Function Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. In the RQC complex, required to promote formation of 'Lys-48'-linked polyubiquitin chains during ubiquitination of incompletely synthesized proteins by LTN1. Also acts as a transcriptional repressor: represses transcription of SRF in vitro and so may play a role in heart development. May play a role in cell death control.
Protein Name Ribosome Quality Control Complex Subunit Tcf25
Nuclear Localized Protein 1
Transcription Factor 25
Tcf-25
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Mainly Nuclear
Alternative Antibody Names Anti-Ribosome Quality Control Complex Subunit Tcf25 antibody
Anti-Nuclear Localized Protein 1 antibody
Anti-Transcription Factor 25 antibody
Anti-Tcf-25 antibody
Anti-TCF25 antibody
Anti-KIAA1049 antibody
Anti-NULP1 antibody
Anti-FKSG26 antibody

Information sourced from Uniprot.org

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