| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TCEB1 (1-112) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ELOC |
| Gene ID: |
6921 |
| Uniprot ID: |
ELOC_HUMAN |
| Immunogen Region: |
1-112 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-112 of human TCEB1 (NP_001191791.1). |
| Immunogen Sequence: |
MDGEEKTYGGCEGPDAMYVK LISSDGHEFIVKREHALTSG TIKAMLSGPGQFAENETNEV NFREIPSHVLSKVCMYFTYK VRYTNSSTEIPEFPIAPEIA LELLMAANFLDC |
| Tissue Specificity | Overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. |
| Post Translational Modifications | Ubiquitinated by the DCX(AMBRA1) complex, leading to its degradation by the proteasome. |
| Function | SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells. Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. This includes the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. As part of a multisubunit ubiquitin ligase complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5.polyubiquitinates monoubiquitinated POLR2A. A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase. |
| Protein Name | Elongin-CElocElongin 15 Kda SubunitRna Polymerase Ii Transcription Factor Siii Subunit CSiii P15Transcription Elongation Factor B Polypeptide 1 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-1234176Reactome: R-HSA-167152Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-180585Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-75955Reactome: R-HSA-8951664Reactome: R-HSA-9010553Reactome: R-HSA-9705462Reactome: R-HSA-983168 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Elongin-C antibodyAnti-Eloc antibodyAnti-Elongin 15 Kda Subunit antibodyAnti-Rna Polymerase Ii Transcription Factor Siii Subunit C antibodyAnti-Siii P15 antibodyAnti-Transcription Elongation Factor B Polypeptide 1 antibodyAnti-ELOC antibodyAnti-TCEB1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
