• Western blot analysis of extracts of various cell lines, using STOM antibody (STJ28455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-STOM antibody (55-288) (STJ28455)

SKU:
STJ28455

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STOM (55-288) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STOM
Gene ID: 2040
Uniprot ID: STOM_HUMAN
Immunogen Region: 55-288
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 55-288 of human STOM (NP_004090.4).
Immunogen Sequence: KIIKEYERAIIFRLGRILQG GAKGPGLFFILPCTDSFIKV DMRTISFDIPPQEILTKDSV TISVDGVVYYRVQNATLAVA NITNADSATRLLAQTTLRNV LGTKNLSQILSDREEIAHNM QSTLDDATDAWGIKVERVEI KDVKLPVQLQRAMAAEAEAS REARAKVIAAEGEMNASRAL KEASMVITESPAALQLRYLQ TLTTIAAEKNSTIVFPLPID MLQGIIGAKHSHLG
Tissue Specificity Detected in erythrocytes (at protein level). Widely expressed.
Function Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.
Protein Name Stomatin
Erythrocyte Band 7 Integral Membrane Protein
Erythrocyte Membrane Protein Band 7.2
Protein 7.2b
Database Links Reactome: R-HSA-2672351
Reactome: R-HSA-6798695
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013409
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Lipid-Anchor
Membrane Raft
Melanosome
Cytoplasmic Vesicle
Localizes To Juxtanuclear Structure Probably Derived From The Golgi Apparatus
Colocalizes With Cortical Actin Microfilaments At Small Plasma Membrane Protrusions
Associates With Alpha-Granular Lipid Rafts
Translocates From The Alpha-Granular Lipid Rafts To The Cell Membrane On Thrombin Activation And Selectively Enriched In Released Microvesicles
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Stomatin antibody
Anti-Erythrocyte Band 7 Integral Membrane Protein antibody
Anti-Erythrocyte Membrane Protein Band 7.2 antibody
Anti-Protein 7.2b antibody
Anti-STOM antibody
Anti-BND7 antibody
Anti-EPB72 antibody

Information sourced from Uniprot.org

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