• Western blot analysis of extracts of various cell lines, using SS18 antibody (STJ29070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SS18 rabbit polyclonal antibody (STJ29070) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using SS18 rabbit polyclonal antibody (STJ29070) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SS18 antibody (1-240) (STJ29070)

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STJ29070

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SS18 (1-240) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SS18
Gene ID: 6760
Uniprot ID: SSXT_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SS18 (NP_001007560.1).
Immunogen Sequence: MSVAFAAPRQRGKGEITPAA IQKMLDDNNHLIQCIMDSQN KGKTSECSQYQQMLHTNLVY LATIADSNQNMQSLLPAPPT QNMPMGPGGMNQSGPPPPPR SHNMPSDGMVGGGPPAPHMQ NQMNGQMPGPNHMPMQGPGP NQLNMTNSSMNMPSSSHGSM GGYNHSVPSSQSMPVQNQMT MSQGQPMGNYGPRPNMSMQP NQGPMMHQQPPSQQYNMPQG GGQHYQGQQPPMGMMGQVN
Tissue Specificity Fairly ubiquitously expressed. Expressed in synovial sarcomas and in other human cell lines. The fusion genes SSXT-SSX1 and SSXT-SSX2 are expressed only in synovial sarcomas.
Function Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation. Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner.
Protein Name Protein Ssxt
Protein Syt
Synovial Sarcoma Translocated To X Chromosome Protein
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein Ssxt antibody
Anti-Protein Syt antibody
Anti-Synovial Sarcoma Translocated To X Chromosome Protein antibody
Anti-SS18 antibody
Anti-SSXT antibody
Anti-SYT antibody

Information sourced from Uniprot.org

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