• Western blot analysis of extracts of various cell lines, using SRP72 antibody (STJ11102732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SRP72 rabbit polyclonal antibody (STJ11102732) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SRP72 rabbit polyclonal antibody (STJ11102732) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SRP72 rabbit polyclonal antibody (STJ11102732) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using SRP72 rabbit polyclonal antibody (STJ11102732) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using SRP72 rabbit polyclonal antibody (STJ11102732) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using SRP72 rabbit polyclonal antibody (STJ11102732) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SRP72 antibody (440-544) (STJ11102732)

SKU:
STJ11102732

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SRP72 (440-544) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRP72
Gene ID: 6731
Uniprot ID: SRP72_HUMAN
Immunogen Region: 440-544
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 440-544 of human SRP72 (NP_008878.3).
Immunogen Sequence: QPKSPAHLSLIREAANFKLK YGRKKEAISDLQQLWKQNPK DIHTLAQLISAYSLVDPEKA KALSKHLPSSDSMSLKVDVE ALENSAGATYIRKKGGKVTG DSQPK
Function Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER. The SRP complex targets the ribosome-nascent chain complex to the SRP receptor (SR), which is anchored in the ER, where SR compaction and GTPase rearrangement drive cotranslational protein translocation into the ER. Binds the signal recognition particle RNA (7SL RNA) in presence of SRP68. Can bind 7SL RNA with low affinity. The SRP complex possibly participates in the elongation arrest function.
Protein Name Signal Recognition Particle Subunit Srp72
Srp72
Signal Recognition Particle 72 Kda Protein
Database Links Reactome: R-HSA-1799339
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Alternative Antibody Names Anti-Signal Recognition Particle Subunit Srp72 antibody
Anti-Srp72 antibody
Anti-Signal Recognition Particle 72 Kda Protein antibody
Anti-SRP72 antibody

Information sourced from Uniprot.org

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