• Western blot analysis of lysate from COLO205, using Synphilin-1 antibody.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of Synphilin-1 antibody in paraffin-embedded lung carcinoma. tissue.
  • Western blot analysis of various cells using Synphilin-1 Polyclonal Antibody

Anti-SNCAIP antibody (797-846 aa) (STJ95857)

SKU:
STJ95857

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Synphilin-1 (797-846 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNCAIP
Gene ID: 9627
Uniprot ID: SNCAP_HUMAN
Immunogen Region: 797-846 aa
Specificity: Synphilin-1 Polyclonal Antibody detects endogenous levels of Synphilin-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Synphilin-1 at the amino acid range 797-846
Post Translational Modifications Ubiquitinated.mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation.
Function Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.
Protein Name Synphilin-1
Sph1
Alpha-Synuclein-Interacting Protein
Database Links Reactome: R-HSA-977225 Q9Y6H5-2
Cellular Localisation Cytoplasm
Detected In Cytoplasmic Inclusion Bodies
Together With Snca
Alternative Antibody Names Anti-Synphilin-1 antibody
Anti-Sph1 antibody
Anti-Alpha-Synuclein-Interacting Protein antibody
Anti-SNCAIP antibody

Information sourced from Uniprot.org

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