| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLU7 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLU7 |
| Gene ID: |
10569 |
| Uniprot ID: |
SLU7_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SLU7 (NP_006416.3). |
| Immunogen Sequence: |
MSATVVDAVNAAPLSGSKEM SLEEPKKMTREDWRKKKELE EQRKLGNAPAEVDEEGKDIN PHIPQYISSVPWYIDPSKRP TLKHQRPQPEKQKQFSSSGE WYKRGVKENSIITKYRKGAC ENCGAMTHKKKDCFERPRRV GAKFTGTNIAPDEHVQPQLM FDYDGKRDRWNGYNPEEHMK IVEEYAKVDLAKRTLKAQKL QEELASGKLVEQANSPKHQW GEEEPNSQMEKDHNSEDED |
| Function | Required for pre-mRNA splicing as component of the spliceosome. Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation. |
| Protein Name | Pre-Mrna-Splicing Factor Slu7Hslu7 |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmPredominantly NuclearShuttling Between The Nucleus And The Cytoplasm Is Regulated By The Cchc-Type Zinc FingerUpon Uv-C Stress StimulusThe Nuclear Concentration Of The Protein DecreasesAffecting Alternative SplicingTranslocates From The Nucleus To The Cytoplasm After Heat Shock Cell TreatmentAccumulates In Cytoplasmic Vesicle-Like Organelles After Heat Shock TreatmentWhich May Represent Stress Granules |
| Alternative Antibody Names | Anti-Pre-Mrna-Splicing Factor Slu7 antibodyAnti-Hslu7 antibodyAnti-SLU7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
